Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9048193818

Category: Medical

Page: 285

View: 404

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer Science & Business Media

ISBN: 9048193826

Category: Medical

Page: 285

View: 660

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum V

Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter B. Gahan

Publisher: Blackwell Pub

ISBN: STANFORD:36105132368627

Category: Science

Page: 345

View: 513

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Circulating Nucleic Acids in Plasma Or Serum

Circulating Nucleic Acids in Plasma Or Serum

This volume, taken from the proceedings of a conference in August 1999, discusses such issues.

Author: Philippe Anker

Publisher:

ISBN: UVA:X004443517

Category: Science

Page: 188

View: 468

Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues.
Categories: Science

Circulating Nucleic Acids in Serum and Plasma CNAPS IX

Circulating Nucleic Acids in Serum and Plasma     CNAPS IX

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 3319824872

Category: Medical

Page: 220

View: 482

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Categories: Medical

Circulating Nucleic Acids in Plasma Or Serum II

Circulating Nucleic Acids in Plasma Or Serum II

This volume reviews and summarizes research regarding the origin, release, clearance and possible functionality of circulating nucleic acids, and points toward future developments in the field.

Author: Y. M. Dennis Lo

Publisher:

ISBN: 1573313785

Category: Science

Page: 295

View: 391

The biology and diagnostic applications of circulating nucleic acids have gained research momentum in the last few years before the publication of this study. In particular, tumour-derived DNA in the plasma or serum of cancer patients has shown promise for the diagnosis and monitoring of many types of cancer, and the discovery of foetal DNA in maternal plasma or serum opens up new possibilities for non-invasive prenatal genetic analysis. This volume reviews and summarizes research regarding the origin, release, clearance and possible functionality of circulating nucleic acids, and points toward future developments in the field.
Categories: Science

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Author: Dave S. B. Hoon

Publisher:

ISBN: UVA:X004817516

Category: Medical

Page: 325

View: 669

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.
Categories: Medical

Annals of the New York Academy of Sciences Circulating Nucleic Acids in Plasma and Serum V

Annals of the New York Academy of Sciences  Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter Gahan

Publisher: Wiley-Blackwell

ISBN: 1573317349

Category: Science

Page: 352

View: 690

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Circulating Nucleic Acids in Plasma and Serum IV

Circulating Nucleic Acids in Plasma and Serum IV

The focus in this volume is on three major applications of the circulating nucleic acids detection method: cancer, fetal medicine, and diseases such as diabetes, stroke, and myocardial infarction.

Author: R. Swaminathan

Publisher: Wiley-Blackwell

ISBN: 157331627X

Category: Science

Page: 372

View: 523

An accurate, inexpensive, and noninvasive method for the early diagnosis of cancer has been something of a holy grail among cancer researchers, but until recently a method meeting all three criteria has been elusive. Nucleic acids were first discovered in circulation in 1948, but it was not until the 1960s and 70s that patients with autoimmune disease and cancer were discovered to have higher levels of circulating DNA than were detected in in healthy persons. The focus in this volume is on three major applications of the circulating nucleic acids detection method: cancer, fetal medicine, and diseases such as diabetes, stroke, and myocardial infarction. In addition, there are reports on the biology and origins of circulating DNA and RNA and on improved methods for the detection of nucleic acids in plasma and serum. The circulating DNA found in cancer patients has many characteristics in common with their tumors, which made it an attractive candidate for use in the diagnosis and management of patients with malignancies. This method can also be applied to the assessment of the sublinical tumor burden in cancer patients, thus reducing the risk of unnecessary chemotherapy. Detection of nucleic acids circulating in maternal serum means that fetal diagnosis may be possible without resorting to the more dangerous and invasive methods now used (e.g., amniotic fluid and chorionic villus sampling). Detection of problems in the pregnancy such as preeclampsia and intrauterine growth retardation may also become possible through maternal blood samples. In diabetic patients, circulating nucleic acids can be used for the early detection of developments such as retinopathy. The benefits of using circulating nucleic acids in the diagnosis and management of cancer and chronic disease will be realized through earlier detection by means of this less expensive and less invasive testing technique and through its potential for closer monitoring of the disease. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring

Circulating Nucleic Acids in Early Diagnosis  Prognosis and Treatment Monitoring

The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9789401791687

Category: Medical

Page: 471

View: 141

DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics
Categories: Medical

Anticancer Research

Anticancer Research

DNA in paired serum and plasma specimens in unselected tumour patients (colorectal cancer, breast cancer, ... 2 Goebel G, Zitt M and Muller HM: Circulating nucleic acids in plasma or serum (CNAPS) as prognostic and predictive markers in ...

Author:

Publisher:

ISBN: NWU:35558005670399

Category:

Page:

View: 180

Categories:

Development and Application of Biomarkers

Development and Application of Biomarkers

Gormally, E., Caboux, E., Vineis, P., and Hainaut, P., Circulating free DNA in plasma or serum as biomarker of carcinogenesis: Practical aspects and biological significance, Mutat. Res. 635, 105–117, 2007. Tsang, J.C. and Lo, Y.M., ...

Author: Roger L. Lundblad

Publisher: CRC Press

ISBN: 1439819807

Category: Medical

Page: 297

View: 193

First introduced to biomedical research in 1980, the term biomarker has taken on a life of its own in recent years and has come to mean a number of things. In biomedical science, biomarker has evolved to most commonly mean a characteristic that can be used either as a diagnostic or a prognostic, but most significantly as a screening indicator for pathologies that tend to be somewhat silent prior to overt clinical display. Applying scientific rigor, as well as a disciplined approach to nomenclature, Roger Lundblad’s Development and Application of Biomarkers rationalizes the current enthusiasm for biomarkers with the use of well-established clinical laboratory analytes in clinical medicine. Highly respected for his work as both a classical protein scientist and as a pioneer in proteomics, Dr. Lundblad catalogs various biomarkers recognized in clinical medicine and, where possible, matches the expectations for advances in screening technologies with the realities of statistical analysis. More specifically, this important reference: Details an extensive list of biomarkers for various stages of a number of cancer types including ovarian, pancreatic, prostate, and breast cancer Looks at how proteomics is used for the discovery and validation of biomarkers Explores the use of microarray technology, ultra-high performance liquid chromatography, and computational bioinformatic approaches for the discovery and use of biomarkers Examines the use of cells and cell fragments as more complex biomarkers Organizes a host of significant biomarkers and essential research by type and use in a series of readily accessible tables Throughout this volume, Dr. Lundblad encourages consideration of biomarkers more as a concept than as laboratory analytes, emphasizing the relation between the discovery of a biomarker and the biology underlying its production. Ultimately, it is a thorough understanding of that underlying biology that will lead to the development of assays that are robust and reproducible, as well as clinically significant.
Categories: Medical

Handbook of Nucleic Acid Purification

Handbook of Nucleic Acid Purification

16.1.1 CELL-FREE DNA IN PLASMA AND SERUM The presence of circulating nucleic acids in blood plasma was first described by Mandel and Métais in 1947 [1], who demonstrated that extracellular nucleic acids could be detected in the ...

Author: Dongyou Liu

Publisher: CRC Press

ISBN: 9781420070972

Category: Science

Page: 584

View: 520

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.
Categories: Science

Circulating Nucleic Acids in Serum and Plasma CNAPS IX

Circulating Nucleic Acids in Serum and Plasma     CNAPS IX

Liquid Profiling of Circulating Nucleic Acids as a Novel Tool for the Management of Cancer Patients Stefan Holdenrieder Abstract Liquid profiling is a traditional concept in laboratory diagnostics using patterns of blood-derived ...

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9783319420448

Category: Medical

Page: 220

View: 124

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Categories: Medical

Advances in Blood Research and Application 2013 Edition

Advances in Blood Research and Application  2013 Edition

However, a small amount of nucleic acids can also be found circulating freely in the blood. The term 'Circulating Nucleic Acids' (CNA) refers to these segments of DNA or RNA found in the bloodstream, plasma, serum or other body fluid.

Author:

Publisher: ScholarlyEditions

ISBN: 9781481694063

Category: Medical

Page: 656

View: 849

Advances in Blood Research and Application / 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Plasma. The editors have built Advances in Blood Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Plasma in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in Blood Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Categories: Medical

Cell Free Nucleic Acids

Cell Free Nucleic Acids

[CrossRef] Pos, O.; Biro, O.; Szemes, T.; Nagy, B. Circulating cell-free nucleic acids: Characteristics and applications. J. Immunol. 2018, 26,937–945. Butt, A.N.; Swaminathan, R. Overview of circulating nucleic acids in plasma/serum.

Author: Bálint Nagy

Publisher: MDPI

ISBN: 9783039280742

Category: Medical

Page: 248

View: 576

The deficits of mammography and the potential of noninvasive diagnostic testing using circulating miRNA profiles are presented in our first review article. Exosomes are important in the transfer of genetic information. The current knowledge on exosome-associated DNAs and on vesicle-associated DNAs and their role in pregnancy-related complications is presented in the next article. The major obstacle is the lack of a standardized technique for the isolation and measurement of exosomes. One review has summarized the latest results on cell-free nucleic acids in inflammatory bowel disease (IBD). Despite the extensive research, the etiology and exact pathogenesis are still unclear, although similarity to the cell-free ribonucleic acids (cfRNAs) observed in other autoimmune diseases seems to be relevant in IBD. Liquid biopsy is a useful tool for the differentiation of leiomyomas and sarcomas in the corpus uteri. One manuscript has collected the most important knowledge of mesenchymal uterine tumors and shows the benefits of noninvasive sampling. Microchimerism has also recently become a hot topic. It is discussed in the context of various forms of transplantation and transplantation-related advanced therapies, the available cell-free nucleic acid (cfNA) markers, and the detection platforms that have been introduced. Ovarian cancer is one of the leading serious malignancies among women, with a high incidence of mortality; the introduction of new noninvasive diagnostic markers could help in its early detection and treatment monitoring. Epigenetic regulation is very important during the development of diseases and drug resistance. Methylation changes are important signs during ovarian cancer development, and it seems that the CDH1 gene is a potential candidate for being a noninvasive biomarker in the diagnosis of ovarian cancer. Preeclampsia is a mysterious disease—despite intensive research, the exact details of its development are unknown. It seems that cell-free nucleic acids could serve as biomarkers for the early detection of this disease. Three research papers deal with the prenatal application of cfDNA. Copy number variants (CNVs) are important subjects for the study of human genome variations, as CNVs can contribute to population diversity and human genetic diseases. These are useful in NIPT as a source of population specific data. The reliability of NIPT depends on the accurate estimation of fetal fraction. Improvement in the success rate of in vitro fertilization (IVF) and embryo transfer (ET) is an important goal. The measurement of embryo-specific small noncoding RNAs in culture media could improve the efficiency of ET.
Categories: Medical