Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9048193818

Category: Medical

Page: 285

View: 366

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer Science & Business Media

ISBN: 9048193826

Category: Medical

Page: 285

View: 494

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.
Categories: Medical

Circulating Nucleic Acids in Serum and Plasma CNAPS IX

Circulating Nucleic Acids in Serum and Plasma     CNAPS IX

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9783319420448

Category: Medical

Page: 220

View: 852

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum V

Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter B. Gahan

Publisher: Blackwell Pub

ISBN: STANFORD:36105132368627

Category: Science

Page: 345

View: 786

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Annals of the New York Academy of Sciences Circulating Nucleic Acids in Plasma and Serum V

Annals of the New York Academy of Sciences  Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter Gahan

Publisher: Wiley-Blackwell

ISBN: 1573317349

Category: Science

Page: 352

View: 600

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Circulating Nucleic Acids in Plasma Or Serum II

Circulating Nucleic Acids in Plasma Or Serum II

This volume reviews and summarizes research regarding the origin, release, clearance and possible functionality of circulating nucleic acids, and points toward future developments in the field.

Author: Y. M. Dennis Lo

Publisher:

ISBN: 1573313785

Category: Science

Page: 295

View: 405

The biology and diagnostic applications of circulating nucleic acids have gained research momentum in the last few years before the publication of this study. In particular, tumour-derived DNA in the plasma or serum of cancer patients has shown promise for the diagnosis and monitoring of many types of cancer, and the discovery of foetal DNA in maternal plasma or serum opens up new possibilities for non-invasive prenatal genetic analysis. This volume reviews and summarizes research regarding the origin, release, clearance and possible functionality of circulating nucleic acids, and points toward future developments in the field.
Categories: Science

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Circulating Nucleic Acids in Plasma serum III and Serum Proteomics

Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Author: Dave S. B. Hoon

Publisher:

ISBN: UVA:X004817516

Category: Medical

Page: 325

View: 789

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.
Categories: Medical

Circulating Nucleic Acids in Plasma and Serum IV

Circulating Nucleic Acids in Plasma and Serum IV

The focus in this volume is on three major applications of the circulating nucleic acids detection method: cancer, fetal medicine, and diseases such as diabetes, stroke, and myocardial infarction.

Author: R. Swaminathan

Publisher: Wiley-Blackwell

ISBN: 157331627X

Category: Science

Page: 372

View: 819

An accurate, inexpensive, and noninvasive method for the early diagnosis of cancer has been something of a holy grail among cancer researchers, but until recently a method meeting all three criteria has been elusive. Nucleic acids were first discovered in circulation in 1948, but it was not until the 1960s and 70s that patients with autoimmune disease and cancer were discovered to have higher levels of circulating DNA than were detected in in healthy persons. The focus in this volume is on three major applications of the circulating nucleic acids detection method: cancer, fetal medicine, and diseases such as diabetes, stroke, and myocardial infarction. In addition, there are reports on the biology and origins of circulating DNA and RNA and on improved methods for the detection of nucleic acids in plasma and serum. The circulating DNA found in cancer patients has many characteristics in common with their tumors, which made it an attractive candidate for use in the diagnosis and management of patients with malignancies. This method can also be applied to the assessment of the sublinical tumor burden in cancer patients, thus reducing the risk of unnecessary chemotherapy. Detection of nucleic acids circulating in maternal serum means that fetal diagnosis may be possible without resorting to the more dangerous and invasive methods now used (e.g., amniotic fluid and chorionic villus sampling). Detection of problems in the pregnancy such as preeclampsia and intrauterine growth retardation may also become possible through maternal blood samples. In diabetic patients, circulating nucleic acids can be used for the early detection of developments such as retinopathy. The benefits of using circulating nucleic acids in the diagnosis and management of cancer and chronic disease will be realized through earlier detection by means of this less expensive and less invasive testing technique and through its potential for closer monitoring of the disease. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science

Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring

Circulating Nucleic Acids in Early Diagnosis  Prognosis and Treatment Monitoring

The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9789401791687

Category: Medical

Page: 471

View: 704

DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics
Categories: Medical

Circulating Nucleic Acids in Plasma Or Serum

Circulating Nucleic Acids in Plasma Or Serum

This volume, taken from the proceedings of a conference in August 1999, discusses such issues.

Author: Philippe Anker

Publisher:

ISBN: 157331269X

Category: Science

Page: 188

View: 579

Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues.
Categories: Science

Anticancer Research

Anticancer Research

The DNA was extracted from 200 ul of plasma using commercial kits based on
affinity columns ( QIAamp Blood Mini ... Finally , the circulating hТERT DNA was
significantly inversely correlated with CA 15.3 serum level ( p = 0.001 ) ( Table I ) .

Author:

Publisher:

ISBN: NWU:35558005724337

Category: Cancer

Page:

View: 932

Categories: Cancer

Handbook of Nucleic Acid Purification

Handbook of Nucleic Acid Purification

16.1.1 CELL-FREE DNA IN PLASMA AND SERUM The presence of circulating nucleic acids in blood plasma was first described by Mandel and Métais in 1947 [1], ...

Author: Dongyou Liu

Publisher: CRC Press

ISBN: 9781420070972

Category: Science

Page: 584

View: 948

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.
Categories: Science

Development and Application of Biomarkers

Development and Application of Biomarkers

Gahan, P.B., Circulating nucleic acids in plasma and serum: Roles in diagnosis and prognosis in diabetes and cancer, Infect. Disord.

Author: Roger L. Lundblad

Publisher: CRC Press

ISBN: 1439819807

Category: Medical

Page: 297

View: 541

First introduced to biomedical research in 1980, the term biomarker has taken on a life of its own in recent years and has come to mean a number of things. In biomedical science, biomarker has evolved to most commonly mean a characteristic that can be used either as a diagnostic or a prognostic, but most significantly as a screening indicator for pathologies that tend to be somewhat silent prior to overt clinical display. Applying scientific rigor, as well as a disciplined approach to nomenclature, Roger Lundblad’s Development and Application of Biomarkers rationalizes the current enthusiasm for biomarkers with the use of well-established clinical laboratory analytes in clinical medicine. Highly respected for his work as both a classical protein scientist and as a pioneer in proteomics, Dr. Lundblad catalogs various biomarkers recognized in clinical medicine and, where possible, matches the expectations for advances in screening technologies with the realities of statistical analysis. More specifically, this important reference: Details an extensive list of biomarkers for various stages of a number of cancer types including ovarian, pancreatic, prostate, and breast cancer Looks at how proteomics is used for the discovery and validation of biomarkers Explores the use of microarray technology, ultra-high performance liquid chromatography, and computational bioinformatic approaches for the discovery and use of biomarkers Examines the use of cells and cell fragments as more complex biomarkers Organizes a host of significant biomarkers and essential research by type and use in a series of readily accessible tables Throughout this volume, Dr. Lundblad encourages consideration of biomarkers more as a concept than as laboratory analytes, emphasizing the relation between the discovery of a biomarker and the biology underlying its production. Ultimately, it is a thorough understanding of that underlying biology that will lead to the development of assays that are robust and reproducible, as well as clinically significant.
Categories: Medical

Nucleic Acid Nanotheranostics

Nucleic Acid Nanotheranostics

DNA in chemotherapy resistant ovarian cancer treated with bevacizumab. ... Circulating nucleic acids in plasma and serum (CNAPS): applications in oncology.

Author: Marco Filice

Publisher: Elsevier

ISBN: 9780128144718

Category: Technology & Engineering

Page: 491

View: 288

Nucleic Acid Nanotheranostics: Biomedical Applications offers a comprehensive overview of improvements and new trends in fabrication of nanostructures as theranostic multifunctional carriers in gene therapy. With a strong focus on medical applications (comprising diagnosis, therapy and imaging), the book also examines gene therapy in an individual patient’s cells or tissues to treat genetic diseases. Sections cover Biomedical and Diagnostic applications of Nucleic Acids, Biologic and Synthetic Advanced Nanostructures for nucleic acid delivery, and important considerations of nanomedicine. This book is a valuable guide for materials scientists, physicians, chemists and engineers, but is also ideal for clinicians wishing to expand their knowledge. Provides a unique source of knowledge (theoretical as well as practical) on nanotheranostic materials for gene therapy at all levels and related scientific areas Covers the pros and cons related to viral and nanomaterial-based delivery of nucleic acids in terms of biosafety, carrier selection, synthesis and bioimaging Presents the only book to include an analysis of nanoformulations approved for clinical use
Categories: Technology & Engineering

Extracellular Nucleic Acids

Extracellular Nucleic Acids

Cortez MA, Calin GA (2009) MicroRNA identification in plasma and serum: a new ... BRAFV600E variant in circulating DNA of stage III-IV melanoma patients.

Author: Yo Kikuchi

Publisher: Springer Science & Business Media

ISBN: 3642126170

Category: Science

Page: 232

View: 451

Extracellular nucleic acids have recently emerged as important players in the fields of biology and the medical sciences. In the last several years, extracellular nucleic acids have been shown to be involved in not only microbial evolution as genetic elements but also to have structural roles in bacterial communities, such as biofilms. Circulating DNA and RNA have been found in human blood and expected to be useful as non-invasive markers for the diagnosis of several diseases. In addition, extracellular nucleic acids have attracted attention as active modulators of the immune system of higher organisms, including humans. This book covers nearly all of the newly developing fields related to extracellular nucleic acids, including those of basic biology, ecology and the medical sciences, and provides readers with the latest knowledge on them.
Categories: Science

Polycystic Kidney Disease

Polycystic Kidney Disease

Jahr, S. et al., DNA fragments in the blood plasma of cancer patients: Quantitations and evidence ... Circulating Nucleic Acids in Plasma and Serum V, 2008.

Author: Jinghua Hu

Publisher: CRC Press

ISBN: 9780429888953

Category: Medical

Page: 346

View: 949

This volume focuses on the investigatory methods applied to autosomal dominant polycystic kidney disease (ADPKD), one of the most common human genetic diseases. ADPKD is caused by mutations in PKD1 and TRPP2, two integral membrane proteins that function as receptor/ion channels in primary cilia of tubular epithelial cells. Thus, ADPKD belongs to ciliopathies, a group of disorders caused by abnormal cilia formation or function. This proposed book will cover the state-of-the-art methods ranging from molecular biology, biochemistry, electrophysiology, to tools in model animal studies. Key Features Explores the role of cilia in polycystic kidney disease Focuses on myriad state-of-the-art methods and techniques Reviews specific mutations integral to this autosomal genetic disease Includes discussions of model systems
Categories: Medical

Annals of the New York Academy of Sciences Circulating Nucleic Acids in Plasma and Serum V

Annals of the New York Academy of Sciences  Circulating Nucleic Acids in Plasma and Serum V

This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter Gahan

Publisher: Wiley-Blackwell

ISBN: 1573317349

Category: Science

Page: 352

View: 338

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member
Categories: Science