orange, and hydroxyurea, all of which may affect both nuclear and organellar RNA and DNA synthesis, have a transition point in the fourth quarter of the cell cycle. The antibiotic cycloheximide, which inhibits cytoplasmic protein ...
Author: Gary Whitson
Nuclear-Cytoplasmic Interactions in the Cell Cycle
REFERENCES  F. Bresciani, in "Cellular Radiation Biology," p. 547, Williams & Wilkins, Baltimore, 1965.  R. Baserga and F. Wiebel, Int. Rev. Exptl. Pathol. 7, 1 (1969). EFFECTS OF HORMONES ON THE CELL CYCLE Olga 1.
2-6 Methods in Cell-Cycle Analysis 1n2n DNA content Figure 2-17 Analysis of cellular DNA content by flow cytometry A large population of haploid budding yeast cells was incubated with a fluorescent DNA dye and then passed through a flow ...
Author: David Morgan
Publisher: New Science Press
The Cell Cycle: Principles of Control provides an engaging insight into the process of cell division, bringing to the student a much-needed synthesis of a subject entering a period of unprecedented growth as an understanding of the molecular mechanisms underlying cell division are revealed.
Müller R . Transcriptional regulation during the mammalian cell cycle . Trends Genet . 1995 ; 11 : 173 - 178 . 155 . La Thangue NB . DP and E2F proteins : components of a heterodimeric transcription factor implicated in cell cycle ...
Author: Patrick J. Daniels
Category: Salivary glands
The sialoadenitis seen in Sjögren's syndrome, an idiopathic, autoimmune exocrinopathy, is characterized by lymphocytic infiltration, acinar cell atrophy, and diminished salivary flow. Increased expressions of laminin, a laminin receptor, and cytokines are also noted. Several in vivo characteristics of the sialoadenitis are also evident in a cytokine-treated salivary gland ductal epithelial cell line. To elucidate mechanisms of salivary gland pathology, the effects of two cytokines, interferon gamma and tumor necrosis factor alpha, on cell proliferation and expressions of basement membrane proteins and alpha-3 integrin were evaluated in cultured salivary gland cells. In cytokine-treated cell monolayers, immunoprecipitation, immunoperoxidase, and Western Blot analysis demonstrated a moderate intracellular accumulation of an immature laminin product, but not fibronectin or collagen IV, concurrent with decreased cell proliferation. Results from RNase Protection assays suggested that the laminin accumulation was unlikely due to increased laminin beta chain gene expression. Furthermore, a significant reduction of glyceraldehyde-3-phosphate dehydrogenase expression was noted with prolonged cytokine treatment, suggesting metabolic defects. To explore the effects of cytokines on acinar cell pathology, cells were grown on Matrigel, where they formed acini with polarized nuclei. Cytokine treatment arrested cells in G1 phase of the cell cycle, as evaluated by flow cytometry, which preceded significant morphological changes and decreased viability. By immunoprecipitation, an altered form of alpha-3 integrin was evident in cultured acinar cells treated with cytokines for prolonged periods, but not in untreated cells. Cytoklines caused no significant changes in laminin expression in acinar cells. From this study, it was evident that the combination of interferon gamma and tumor necrosis factor alpha resulted in a block in G1 phase for acinar cells. This cell cycle arrest occurred prior to accumulation of the alpha-3 integrin variant or significant degenerative cellular changes. Information from the present and previous studies suggest that cytokines may alter adhesion and block cell cycle progression in acinar cells in Sjögren's syndrome sialoadenitis. Further studies may help elucidate how these cytokine-mediated cellular changes contribute to acinar cell death.
Weinberg, R. A. (1995) The retinoblastoma protein and cell cycle control. Cell. 81:323-330. Duriez, C., Falette, N., Audoynaud, C., Moyret-Lalle, C., Bensaad, K., Courtois, S., Wang, Q., Soussi, T., and Puisieux, A. (2002) The human ...
Imaginal cells arrest in G1 during mid - embryogenesis and require the influx of nutrients from larval feeding to reactivate their cell cycle . Their first S phase occurs after a substantial ( 6x ) increase in cell mass ( Madhavan ...
Author: Gregory R. Bock
Publisher: John Wiley and Sons
This book brings together scientists working at the interface between the cell cycle, cell growth and development in a variety of model systems and research paradigms. The focus is on understanding how such diverse developmental inputs can modulate cell cycle regulation and, reciprocally, how a common way of regulating cell cycle progression can participate in different developmental strategies.
As a consequence of rule 5 it is expected that the rate of cell division will remain unchanged for approximately 60 min after an asynchronous population changes from one volume growth rate to another . Thus average cell volume will ...
This book focuses on the intersection between cell cycle regulation and embryo development.
Author: Jacek Z. Kubiak
Publisher: Springer Science & Business Media
This book focuses on the intersection between cell cycle regulation and embryo development. Specific modifications of the canonical cell cycle occur throughout the whole period of development and are adapted to fulfil functions coded by the developmental program. Deciphering these adaptations is essential to comprehending how living organisms develop. The aim of this book is to review the best-known modifications and adaptations of the cell cycle during development. The first chapters cover the general problems of how the cell cycle evolves, while consecutive chapters guide readers through the plethora of such phenomena. The book closes with a description of specific changes in the cell cycle of neurons in the senescent human brain. Taken together, the chapters present a panorama of species - from worms to humans - and of developmental stages - from unfertilized oocyte to aged adult.