The Primary Structure of Transfer RNA

The Primary Structure of Transfer RNA

This is one of the most urgent problems in molecular biology, for until it is solved it is im possible to understand fully the mechanisms of fundamental living processes.

Author: Tatʹi︠a︡na Vladimirovna Venkstern

Publisher: Springer

ISBN: WISC:89034766949

Category: Science

Page: 322

View: 268

The study of the primary structure of nucleic acids, i. e. , the determination of the nucleotide sequence in ribosomal, transfer, and messenger types of RNA and in DNA, is an essential preliminary to the attempt to correlate the structures of these compounds with their functions. This is one of the most urgent problems in molecular biology, for until it is solved it is im possible to understand fully the mechanisms of fundamental living processes. Research has naturally tended to concentrate on the nucleic acids of smallest molecular weight, namely the transfer RNAs, and tremendous progress has been made in recent years in the unraveling of their structure. In 1958-1959, when structural investigation was still in its infancy, it was even doubted whether the structure of any transfer RNA could be deter mined. No methods of isolating homogeneous preparations were available, and it was uncertain whether the very slight physical and chemical differences between tRNAs of different specificity would be sufficient to allow successful fractionation. When the first enriched preparations were obtained, there arose the problem of whether it was possible to determine the nucleotide sequence having regard to the limited number of heterogeneous structural elements present in tRNA. These doubts were resolved in 1965, when the structure of the first nucleic acid, alanine-specific transfer RNA, was unraveled in Holley's laboratory.
Categories: Science

The Primary Structure of Transfer RNA

The Primary Structure of Transfer RNA

This is one of the most urgent problems in molecular biology, for until it is solved it is im possible to understand fully the mechanisms of fundamental living processes.

Author: Tat Yana Venkstern

Publisher: Springer

ISBN: 1468419730

Category: Science

Page: 296

View: 622

The study of the primary structure of nucleic acids, i. e. , the determination of the nucleotide sequence in ribosomal, transfer, and messenger types of RNA and in DNA, is an essential preliminary to the attempt to correlate the structures of these compounds with their functions. This is one of the most urgent problems in molecular biology, for until it is solved it is im possible to understand fully the mechanisms of fundamental living processes. Research has naturally tended to concentrate on the nucleic acids of smallest molecular weight, namely the transfer RNAs, and tremendous progress has been made in recent years in the unraveling of their structure. In 1958-1959, when structural investigation was still in its infancy, it was even doubted whether the structure of any transfer RNA could be deter mined. No methods of isolating homogeneous preparations were available, and it was uncertain whether the very slight physical and chemical differences between tRNAs of different specificity would be sufficient to allow successful fractionation. When the first enriched preparations were obtained, there arose the problem of whether it was possible to determine the nucleotide sequence having regard to the limited number of heterogeneous structural elements present in tRNA. These doubts were resolved in 1965, when the structure of the first nucleic acid, alanine-specific transfer RNA, was unraveled in Holley's laboratory.
Categories: Science

The Primary Structure of Transfer RNA

The Primary Structure of Transfer RNA

During the study of primary structure, yet another advantage of tr{NA was discovered. This was that the tRNA molecule has a rigid secondary structure; because of this structure, it is possible to obtain products of incomplete enzymic ...

Author: Tat Yana Venkstern

Publisher: Springer Science & Business Media

ISBN: 9781468419719

Category: Science

Page: 296

View: 870

The study of the primary structure of nucleic acids, i. e. , the determination of the nucleotide sequence in ribosomal, transfer, and messenger types of RNA and in DNA, is an essential preliminary to the attempt to correlate the structures of these compounds with their functions. This is one of the most urgent problems in molecular biology, for until it is solved it is im possible to understand fully the mechanisms of fundamental living processes. Research has naturally tended to concentrate on the nucleic acids of smallest molecular weight, namely the transfer RNAs, and tremendous progress has been made in recent years in the unraveling of their structure. In 1958-1959, when structural investigation was still in its infancy, it was even doubted whether the structure of any transfer RNA could be deter mined. No methods of isolating homogeneous preparations were available, and it was uncertain whether the very slight physical and chemical differences between tRNAs of different specificity would be sufficient to allow successful fractionation. When the first enriched preparations were obtained, there arose the problem of whether it was possible to determine the nucleotide sequence having regard to the limited number of heterogeneous structural elements present in tRNA. These doubts were resolved in 1965, when the structure of the first nucleic acid, alanine-specific transfer RNA, was unraveled in Holley's laboratory.
Categories: Science

X ray Studies on the Three dimensional Structure of Transfer RNA

X ray Studies on the Three dimensional Structure of Transfer RNA

The structure of transfer RNA was a topic of scientific discussion even before the molecule was known to exist. Shortly after the elucidation of the chemical structure of DNA and its implication as the information molecule which directs ...

Author: Mindaugas Paul Labanauskas

Publisher:

ISBN: WISC:89010822955

Category:

Page: 240

View: 951

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Handbook of Genetics

Handbook of Genetics

Kowalski, S., T. Yamane, and J. R. Fresco, 1971 Nucleotide sequence of the “denaturable” leucine transfer RNA from yeast. Science 172:385–387. ... Merril, C. R., 1968 Reinvestigation of the primary structure of yeast alanine tRNA.

Author: Robert King

Publisher: Springer Science & Business Media

ISBN: 9781461571483

Category: Medical

Page: 668

View: 997

Many modern geneticists attempt to elucidate the molecular basis of phenotype by utilizing a battery of techniques derived from physical chemistry on subcellular components isolated from various species of organisms. Volume 5 of the Handbook of Genetics provides explanations of the advantages and shortcomings of some of these revolutionary tech niques, and the nonspecialist is alerted to key research papers, reviews, and reference works. Much of the text deals with the structure and func tioning of the molecules bearing genetic information which reside in the nucleus and with the processing of this information by the ribosomes resid ing in the cytoplasm of eukaryotic cells. The mitochondria, which also live in the cytoplasm of the cells of all eukaryotes, now appear to be separate little creatures. These, as Lynn Margulis pointed out in Volume 1, are the colonial posterity of migrant prokaryotes, probably primitive bacteria that swam into the ancestral precursors of all eukaryotic cells and remained as symbionts. They have maintained themselves and their ways ever since, replicating their own DNA and transcribing an RNA quite different from that of their hosts. In a similar manner, the chloroplasts in all plants are self-replicating organelles presumably derived from the blue-green algae, with their own nucleic acids and ribosomes. Four chapters are devoted to the nucleic acids and the ribosomal components of both classes of these semi-independent lodgers. Finally, data from various sources on genetic variants of enzymes are tabulated for ready reference, and an evaluation of this information is attempted.
Categories: Medical

Progress in Biophysics and Molecular Biology

Progress in Biophysics and Molecular Biology

THE STRUCTURES AND FUNCTIONS OF TRANSFER RNA JOHN P. GODDARD Department of Biochemistry, University of Glasgow, Glasgow G12 800, Scotland CONTENTS I. ... Determined trNA primary structures and their common secondary structure 239 III.

Author: J. A. V. Butler

Publisher: Elsevier

ISBN: 9781483144412

Category: Science

Page: 328

View: 921

Progress in Biophysics and Molecular Biology, Volume 32 summarizes the significant progress that has been made in the fields of biophysics and molecular biology. Topics range from metabolic regulation and transfer RNA to cellular metabolism and prokaryotic and eukaryotic ribosomes. This volume consists of five chapters and begins with a discussion of mathematical models used in the study of metabolic regulation, with emphasis on the energy metabolism of eukaryotes. The next chapter examines the possible functions of transfer RNA minor components, paying particular attention to the principle of location-function relationships. The reader is also introduced to spatial-functional correlations in cellular metabolism and highlights the role of organize multienzyme systems, along with the fundamentals of ribosome structure and function in prokaryotes and eukaryotes. A chapter that analyzes the structures and functions of transfer RNA concludes the book. This book will be of interest to scientists, students, and researchers working in the fields of biophysics and molecular biology.
Categories: Science

Structural RNA Synthetic and Unannotated Sequences

Structural RNA  Synthetic  and Unannotated Sequences

[tRNA] EMBL ID: BSF ACCESSION NUMBERS: K00332 DATE: entered 84-01-04 REFERENCES: [l] (bases l to 76) Keith,G.. ... "The primary structure of Phe-tRNA from Bacillus stearothermophilus"; FEBS Lett 60, 286-289 (1975) KEYWORDS: transfer RNA ...

Author: Edwin J. Atencio

Publisher: Elsevier

ISBN: 9781483218847

Category: Science

Page: 602

View: 255

Nucleotide Sequences 1986/1987, Volume VII: Structural RNA, Synthetic, and Unannotated Sequences presents data that reflect the information found in GenBank Release 44.0 of August 1986. This book provides information pertinent to the unique international collaboration between two leading nucleotide sequence data libraries, one based in Europe and one in the United States. Organized into three sections, this volume begins with an overview of the sequences, some basic identifying information, and some of the biological annotations. This text then discusses the EMBL Nucleotide Sequence Data Library, an international center of fundamental research with its main focus in the fields of cell biology, molecular structures, instrumentation, and differentiation. This book discusses as well the GenBank database established in 1982 by the National Institute of General Medical Sciences (NIGMS) of the U.S National Institutes of Health (NIH). This book is a valuable resource for molecular biologists and other investigators collecting the large number of reported DNA and RNA sequences and making them available in computer-readable form.
Categories: Science

The Genetic Mechanism and the Origin of Life

The Genetic Mechanism and the Origin of Life

Primary structure of a methionine tRNA from E. coli. Nature 220:1039–1040. * Dube, S. K., and Marcker, K. A. 1969. The nucleotide sequence of N-formyl-methionyl-transfer RNA. Eur, J. Biochem. 8:256–262. Dube, S. K., Marcker, K. A., ...

Author: Lawrence Dillon

Publisher: Springer Science & Business Media

ISBN: 9781468424362

Category: Science

Page: 563

View: 776

As shown in the text, there can be little doubt that the genetic mechanism is, for all practical purposes, equivalent to life itself. Consequently, it is unrealistic to seek knowledge of the origin of life and its subsequent evolution without si multaneously searching for an understanding of how this apparatus arose and evolved. Fortunately, the annual publication over the recent years of thousandS" of papers dealing with the genetic processes has brought the state of knowledge to a level where a synthesis of their major details in relation to life's history is feasible. Because of the voluminous body of literature, no single book can pos sibly treat all the ramifications of this fundamental subject; subdivision into multiple volumes is necessary. This volume, the first of a trilogy, explores the molecular aspects of the problem in connection with the precellular aspects up to the point of the origin of the cell. The second, currently in progress, is con cerned with the subsequent evolution of the cell as revealed by the energy related organelles and their genetic apparatuses and by ultrastructural details of other cellular parts. The third volume, as presently planned, deals with devel opmental, immunological, and other complexities at the organismic level and, in so doing, throws additional light on basic properties of the genetic processes themselves. Thus, the genetic apparatus provides the warp, and evolution the woof, of the intricate fabric that emerges.
Categories: Science

Nucleic Acids In Plants

Nucleic Acids In Plants

Sodd , M. A. , Analysis of the primary and secondary structure of tRNA , in Handbook of Biochemistry and Molecular Biology , Vol . 2 , 3rd ed . , Fasman , G. D. , Ed . , CRC Press , Cleveland , 1976 , 423 . 172.

Author: Timothy C. Hall

Publisher: CRC Press

ISBN: 9781351083546

Category: Science

Page: 269

View: 393

Our ambition in the organization of this book was to explore the current stus of knowledge about nucleic acids in plants. We wanted the reader to be able to learn how this research is being undertaken. Therefore, we asked the contributing authors to include details of approaches and methods. Where feasible, the have provided protocols that can be followed by those who wish to repeat results, extend data, make improvements, or use them in new applications.
Categories: Science

Cumulated Index Medicus

Cumulated Index Medicus

R The primary structure of E. coli tRNA Ile minor recognizing beta - D - arabinofuranosyladenine triphosphate on tRNA Organization of tRNA and rRNA genes in N. crassa AUA codon . Kuchino Y , et al . and 5 S RNA synthesis in isolated ...

Author:

Publisher:

ISBN: OSU:32436001304052

Category: Medicine

Page:

View: 451

Categories: Medicine